Analysis of Asn aminoacyl synthetase.

Analysis performed on 2024-09-30 10:19:57

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 19361 256 19105 12053
Vertebrata 1443 273 1170 474
Archaea 1395 60 1335 408
Mammals 557 94 463 129

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
548 15 476

Domains

Domains
Current Study 1, 111
Guo et al (2010) 1, 99

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 438 110 548 3.981818
Number of Variants 413 78 491 5.294872
Number of Pathogenic Variants 12 3 15 4.000000
Number of Benign/Unknown Variants 401 75 476 5.346667
Fisher exact test 0.716963

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 449 99 548 4.535354
Number of Variants 423 68 491 6.220588
Number of Pathogenic Variants 12 3 15 4.000000
Number of Benign/Unknown Variants 411 65 476 6.323077
Fisher exact test 0.448292

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 438 110 0.90 (0.01) 0.88 (0.01) 9.327e-01 9.389e-01 25551.50 1.62e-01
Guo et al (2010) 449 99 0.90 (0.01) 0.88 (0.01) 9.325e-01 9.433e-01 23050.50 2.82e-01

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains